Ca2+-stimulated adenylyl cyclase isoform AC1 is preferentially expressed in guinea-pig sino-atrial node cells and modulates theIfpacemaker current
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چکیده
منابع مشابه
Connexin 30 is expressed in the mouse sino-atrial node and modulates heart rate.
AIMS This study aimed at characterizing expression and the functional role of the Gjb6 gene, encoding for connexin 30 (Cx30) protein, in the adult mouse heart. METHODS AND RESULTS The expression of the Gjb6 gene in the mouse heart was investigated by RT-PCR and sequencing of amplified cDNA fragments. The sites of Gjb6 expression were identified in the adult heart using transgenic mice with re...
متن کاملCa2+-dependent regulation of guinea pig brain adenylate cyclase.
The effect of Ca2+ on guinea pig bra in adenylate cyclase has been studied using metal buffers to regulate the free Ca2+ concentration. Particulate fractions prepared in the presence of CaZ+ (to bind all of the available calmodulin to all of the available binding sites) exhibited a biphasic response to Ca2+. The first was a 2fold stimulation of basal cyclase activity (half-maximal = 0.08 p ~ ) ...
متن کاملAdenylyl cyclase isoform v mediates renin release from juxtaglomerular cells.
We have shown previously that decreasing intracellular calcium in the juxtaglomerular cells increases both cAMP formation and renin release. We hypothesized that this is because of an interaction between intracellular calcium and the calcium-inhibitable isoform of adenylyl cyclase, type-V. We used primary cultures of juxtaglomerular cells isolated from C-57/B6 mice at 70% to 80% confluence. Wes...
متن کاملLocalisation and functional significance of ryanodine receptors during beta-adrenoceptor stimulation in the guinea-pig sino-atrial node.
OBJECTIVE Recent evidence shows that calcium released from the sarcoplasmic reticulum (SR) plays an important role in the regulation of heart rate. The aim of this study was to investigate the subcellular distribution of ryanodine receptors in the guinea-pig sino-atrial (SA) node and to determine their functional role in the regulation of pacemaker frequency in response to beta-adrenoceptor sti...
متن کاملArrhythmia/Electrophysiology Ca -Stimulated Adenylyl Cyclase AC1 Generates Efficient Biological Pacing as Single Gene Therapy and in Combination With HCN2
Background—Biological pacing performed solely via HCN2 gene transfer in vivo results in relatively slow idioventricular rates and only moderate autonomic responsiveness. We induced biological pacing using the Ca -stimulated adenylyl cyclase AC1 gene expressed alone or in combination with HCN2 and compared outcomes with those with single-gene HCN2 transfer. Methods and Results—We implanted adeno...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 2007
ISSN: 0022-3751
DOI: 10.1113/jphysiol.2007.133439